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MEMSnet Home: MEMS-Talk: Information needed regarding Dye cocentration vs Intensity
Information needed regarding Dye cocentration vs Intensity
2006-03-20
Vishwanath Somashekar
2006-03-20
Gareth Jenkins
2006-03-20
Lee Pang
2006-03-20
Martijn van Duijn
Information needed regarding Dye cocentration vs Intensity
Martijn van Duijn
2006-03-20
I agree with the photobleaching diagnosis. Except for reducing the
excitation light with ND filters, you could also try to get rid of
oxygen in your solution, either by bubbling N2 before use, or by using
vacuum for a while. Oxygen mediates the photobleaching, if you get rid
of it, things work a lot better.
You can also look into the bag of tricks from microscopy; antioxidant
additives like ascorbic acid or propyl gallate may help. There are also
several oxygen scavenging mixes available that continuously eat up O2 in
your solution.

Martijn

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