Is this NMR frequencies, Infrared absorption, UV absorption, Visual
absorption?

What you do is purchase or have the equipment to measure the spectra of
interest. Then you dissolve the sample in the solvent of your choice and
pour some of it in the cell. You will want to make sure you have a known
concentration. Then you have the machine read it, store it. Rerun with
the other solvent and compare spectra.

Proteins will have infrared spectra relating to their amino acids, but
not really anything much specific to the whole protein for Infrared. Of
course if you are doing NMR at very high field strengths, you would get
spectra unique to each hydrogen for small proteins.

Ed

-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of saravan kallempudi
Sent: Wednesday, November 26, 2008 7:28 AM
To: [email protected]
Subject: [mems-talk] how to find excitation frequencies of protein
moleculesin solvents like PBS and H2O

Hello all,

I want to measure the frequencies of excitation of organic compounds in
various solvents.
As we know that with protein interactions there will be change in their
dielctric properties and I want to read the excitation frequencies.
Can anyone suggest any method for the above.

Thanks,
Saravan